KLH-conjugated synthetic peptide of CSD2 derived from Arabidopsis thaliana AT2G28190.
Protein A purified
Reconstitution with 150 µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Lane 1: 10μg total protein from Arabidopsis thaliana leaf. Lane 2: 10μg total protein from Zea mays leaf. Lane 3: 50μg total protein from Zea mays leaf. Lane 4: 10μg total protein from Glycine max leaf. Lane 5: 50μg total protein from Glycine max leaf. Lane 6: 10μg total protein from Oryza sativa leaf. Lane 7: 50μg total protein from Oryza sativa leaf. Lane 8: 50μg total protein from Hordeum vulgare leaf. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.
Western Blot (1:500-1:1000) Note: Optimal dilutions/concentrations should be determined by the end user.
22 / 16 kDa
Glycine max, Oryza sativa, Hordeum vulgare
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Arabidopsis thaliana, Brassica napus, Brassica rapa,and 80-99% homologues with the sequence inSolanum tuberosum, Cucumis sativus, Vitis vinifera, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Gossypium raimondii, Medicago truncatula, Zea mays, Triticum aestivum. The sequence of the synthetic peptide used for immunization is 80% homologues with the sequence in CSD1 (AT1G08830). For more species homologues information, please contact tech support at email@example.com.