Catalase is a tetrameric iron porphyrin that catalyses the dismutation of H2O2 to water and oxygen. The Arabidopsis genome encodes three catalase subunits, CAT1 (AT1G20630), CAT2 (AT4G35090), and CAT3 (AT1G20620). These three individual subunits associate to form at least six catalase isoforms.
Cat 2, CATALASE 2, CAT2, Catalase-2
KLH-conjugated synthetic peptide of Cat 2 derived from Arabidopsis thaliana AT4G35090.
Immunogen affinity purified
Reconstitution with 150 µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
57 / 53 kDa
Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Brassica napus, and 80-99% homologues with the sequence in Cucumis sativus, Vitis vinifera, Solanum lycopersicum, Solanum tuberosum, Nicotiana tabacum. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 15 μg total protein from Arabidopsis thaliana. Lane 2: 30 μg total protein from Arabidopsis thaliana. Lane 3: 15 μg cytosolic protein from Arabidopsis thaliana. Lane 4: 30 μg cytosolic protein from Arabidopsis thaliana. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.