ASY1 / Anti-Meiosis-specific protein ASY1 antibody
Rabbit polyclonal antibody
ASY1 protein is initially distributed as numerous foci throughout the chromatin. It is required for normal meiosis in male and female gametophytes. And it plays a crucial role in coordinating the activity of DMC1, a key member of the homologous recombination machinery.
ASY1, ASYNAPTIC 1, ATASY1
KLH-conjugated synthetic peptide derived from C-terminal section of ASY1 protein in Arabidopsis thaliana AT1G67370.
Immunogen affinity purified
Reconstitution with 150µl of sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica rapa, Brassica napus. For more species homologues information, please contact tech support at email@example.com.
Lane 1: 3.75 μg nuclear protein from Arabidopsis thaliana leaf. Lane 2: 7.5 μg nuclear protein from Arabidopsis thaliana leaf. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.