Annexin (ANN) is a member of a diverse, multigene family of calcium-dependent transporters. There are eight annexins in arabidopsis: ANN1 (AT1G35720), ANN2 (AT5G65020), ANN3 (AT2G38760), ANN4 (AT2G38750), ANN5 (AT1G68090), ANN6 (AT5G10220), ANN7 (AT5G10230), ANN8 (AT5G12380).
ANN1, ANNAT1, ANNEXIN 1, ATANN1, ATOXY5, OXY5
KLH-conjugated synthetic peptide of ANN1 derived from Arabidopsis thaliana AT1G35720.
Immunogen affinity purified
Reconstitution with 150 µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa. For more species homologues information, please contact tech support at firstname.lastname@example.org.
20 μg total protein from Arabidopsis thaliana. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.