COX3 / Anti-Cytochrome c oxidase subunit COX3, mitochondrial antibody
Rabbit polyclonal antibody
Cytochrome c oxidase is the last enzyme in the respiratory electron transport chain of mitochondria and it is also called Complex IV. Cytochrome c oxidase receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In higher plants mitochondria, Complex IV processes 14 subunits. COX3 (ATMG00730) is one subunit of the Complex IV.
COX3, CYTOCHROME C OXIDASE SUBUNIT 3
KLH-conjugated synthetic peptide of COX3 derived from Arabidopsis thaliana ATMG00730, AT2G07687.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
30 / 26 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Vitis vinifera, Glycine max, Brassica rapa subsp. oleifera, and 80-99% homologues with the sequence in Hordeum vulgare subsp. vulgare, Oryza sativa Japonica Group, Hordeum vulgare subsp. spontaneum, Triticum aestivum, Zea mays subsp. mays, Brassica napus, Populus trichocarpa, Cucumis sativus, Gossypium raimondii. For more species homologues information, please contact tech support at firstname.lastname@example.org.
7.5 μg mitochondria protein from Arabidopsis thaliana leaf. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.