Mitochondrial F0F1-ATP synthase is also called Complex V and it synthesis ATP from ADP and Pi using the proton motive force created by respiratory electron transport. ATP1 (ATMG01190/AT2G07698) is a subunit of mitochondrial F0F1-ATP synthase in Arabidopsis.
ATP1, ATP SYNTHASE SUBUNIT 1
KLH-conjugated synthetic peptide of ATP1 derived from Arabidopsis thaliana ATMG01190, AT2G07698.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
55 kDa (ATMG01190), 86 kDa (AT2G07698)
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. oleifera, and 80-99% homologues with the sequence in Triticum aestivum, Spinacia oleracea, Zea mays. For more species homologues information, please contact tech support at email@example.com.
Lane 1: 8 μg mitochondria protein from Arabidopsis thaliana leaf. Lane 2: 80 μg total protein from Arabidopsis thaliana leaf. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.