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ATP4 / Anti-ATP synthase subunit 4, mitochondrial antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0587S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Mitochondrial F0F1-ATP synthase is also called Complex V and it synthesis ATP from ADP and Pi using the proton motive force created by respiratory electron transport. ATP4 (ATMG00640) is a subunit of mitochondrial F0F1-ATP synthase in Arabidopsis.

  Synonyms:

ATP4, ORF25

  Immunogen:

KLH-conjugated synthetic peptide of ATP4 derived from Arabidopsis thaliana ATMG00640.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

22 / 20 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Brassica rapa, Brassica napus, Solanum tuberosum, Nicotiana tabacum, Spinacia oleracea, Solanum lycopersicum, Medicago truncatula, Glycine max, Gossypium raimondii, and 80-99% homologues with the sequence in Triticum aestivum.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

7.5 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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