Mitochondrial F0F1-ATP synthase is also called Complex V and it synthesis ATP from ADP and Pi using the proton motive force created by respiratory electron transport. ATP4 (ATMG00640) is a subunit of mitochondrial F0F1-ATP synthase in Arabidopsis.
KLH-conjugated synthetic peptide of ATP4 derived from Arabidopsis thaliana ATMG00640.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:5000) Note: Optimal dilutions/concentrations should be determined by the end user.
22 / 20 kDa
Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Brassica rapa, Brassica napus, Solanum tuberosum, Nicotiana tabacum, Spinacia oleracea, Solanum lycopersicum, Medicago truncatula, Glycine max, Gossypium raimondii, and 80-99% homologues with the sequence in Triticum aestivum. For more species homologues information, please contact tech support at email@example.com.
7.5 μg mitochondria protein from Arabidopsis thaliana leaf. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:5000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.