The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity). Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.
Gamma-COP, Gamma-coat protein
KLH-conjugated synthetic peptide of Gamma-COP derived from Arabidopsis thalianaAT4G34450.
Protein A purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, and 80-99% homologues with the sequence in Gossypium raimondii, Brassica rapa. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 21 μg soluble protein from Arabidopsis thaliana leaf. Lane 2: 41 μg soluble protein from Arabidopsis thaliana leaf. Lane 3: 82 μg soluble protein from Arabidopsis thaliana leaf. Electrophoresis: 10% SDS –PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.