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PetA / Anti-Cytochrome f antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0022S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Cytochrome f is the largest subunit of cytochrome b6f complex. In its structure and functions, the cytochrome b6f complex bears extensive analogy to the cytochrome bc1 complex of mitochondria and photosynthetic purple bacteria. Cytochrome f(cyt f) plays a role analogous to that of cytochrome c1, in spite of their different structures. Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin.

  Synonyms:

PetA, Cytochrome f protein ( PetA) of thylakoid Cyt b6/f-complex

  Immunogen:

KLH-conjugated synthetic peptide of PetA derived from Chlamydomonas reinhardtii.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

34 / 33 kDa

  Confirmed Reactivity: 

Chlamydomonas reinhardtii

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 10 μg total protein from WT of Chlamydomonas reinhardtii.
Lane 2: 10 μg total protein from mutant of Chlamydomonas reinhardtii.
Lane 3: 20 μg total protein from WT of Chlamydomonas reinhardtii.
Lane 4: 20 μg total protein from mutant of Chlamydomonas reinhardtii.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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