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Trx / Anti-Trx tag antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY5015
数量:999
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Product Information

  Description:

Mouse monoclonal antibody

  Synonyms:

Trx tag

  Immunogen:

Recombinant protein corresponding to fragment (aa/1-111) Trx Tag.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Confirmed Reactivity: 

Anti-Trx tag recognizes Trx-tagged fusion proteins.

 

Application Example

Lane 1: 0.1 ng Trx-tagged fusion protein.
Lane 2: 0.5 ng Trx-tagged fusion protein. 
Lane 3: 1 ng Trx-tagged fusion protein. 
Lane 4: 2.5 ng Trx-tagged fusion protein.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006). 
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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