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Cry1A / Anti-Cry1A antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0621
数量:999
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Product Information

  Description:

Mouse monoclonal antibody

  Background:

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat. It promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

  Synonyms:

Cry1A

  Immunogen:

Synthetic peptide of Cry1Ab/1Ac/1Ah/1C serial protein from Bacillus thuringiensis.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

60-70 kDa

  Confirmed Reactivity: 

Zea mays

  Predicted Reactivity: 

Anti-Cry1A recognizes recombinant Cry1Ab/1Ac/1Ah/1C protein and transgenic rice.

 

Application Example

Lane 1: 2.5 µg total soluble protein from transgenic Zea mays leaf. 
Lane 2: 5 µg total soluble protein from transgenic Zea mays leaf. 
Lane 3: 10 µg total soluble protein from transgenic Zea mays leaf. 
Lane 4: 20 µg total soluble protein from transgenic Zea mays leaf. 
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006).
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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