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LjHO1 / Anti-Heme oxygenase 1 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1714S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Lj1g3v0672150.1.

  Synonyms:

LjHO1 (Lotus japonicus heme oxygenase1)

  Immunogen:

Recombinant protein of His-LjHO1△TP.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

33 kDa

  Confirmed Reactivity: 

Anti-LjHO1 recognizes LjHO1△TP or full length LjHO1 proteins or LjHO1 proteins fused with tag.

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 30 μg total Nicotiana benthamiana leaf protein. 
Lane 2: 30 μg total Arabidopsis thaliana leaf protein.
Lane 3: 30 μg total Glycine max leaf protein. 
Lane 4: 30 μg total Chlamydomonas reinhardtii protein. 
Lane 5: 30 μg total Lotus japonicus protein.
Lane 6: 5 ng GST-LjHO1 fusion protein.
Lane 7: 1 ng GST-LjHO1 fusion protein.
Lane 8: 0.1 ng GST-LjHO1 fusion protein.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to PVDF membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1500 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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