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PKG / Anti-cGMP-dependent protein kinase foraging protein antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1718
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

cGMP-dependent protein kinase (PKG), encoded by foraging, has two natural allelic variants and has previously been implicated in several important physiological characteristics including: foraging patterns, learning and memory, and environmental stress tolerance.

  Synonyms:

PKG

  Immunogen:

KLH-conjugated synthetic peptide of PKG derived from Mythimna separata D0EJV9.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.



 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

 68 / 75 kDa

  Confirmed Reactivity: 

Mythimna separata

  Predicted Reactivity:  

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 20 μg Total protein of Mythimna Separata’s head. 
Lane 2: 80 μg Total protein of Mythimna Separata’s head. 
Lane 3: 20 μg Total protein of Mythimna Separata’s body. 
Lane 4: 80 μg Total protein of Mythimna Separata’s body. 
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃. 
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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