返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体 藻类抗体
GlgB / Anti-1,4-alpha-glucan branching enzyme GlgB antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY5155S
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

sll0158

  Synonyms:

GlgB, BE

  Immunogen:

KLH-conjugated synthetic peptide of glgB protein derived from Synechocystis sp. PCC 6803 (sll0158)

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

90 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

WT: 50 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1.5h.
Blocking: 5% skim milk at RT or 4℃ for 2h.
Primary antibody: 1:100 dilution overnight at 4℃.
Secondary antibody: 1:6000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


猜你喜欢