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glgp / Anti-Alpha-1,4 glucan phosphorylase antibody
价格:¥2860.00 ¥3180.00
积分:150 分
货号:PHY5168A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

slr1367

  Synonyms:

glgp

  Immunogen:

KLH-conjugated synthetic peptide of glgp protein derived from Synechocystis sp. PCC 6803 (slr1367)

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

98 / 95 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For homologues with other species especially algae, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 10 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803.
Lane 2: 20 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1.5 h.
Blocking: 5% skim milk at RT or 4℃ for 2 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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