KLH-conjugated synthetic peptide of pgk protein derived from Synechocystis sp. PCC 6803 (slr0394)
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Synechocystis sp. PCC 6803
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Arabidopsis thaliana, Glycine max, Spinacia oleracea, Oryza sativa Japonica Group, Zea may. For more species homologues information, please contact tech support at firstname.lastname@example.org.
50 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1.5h. Blocking: 5% skim milk at RT or 4℃ for 2h. Primary antibody: 1:300 dilution overnight at 4℃. Secondary antibody: 1:6000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.