α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. It can hydrolyze alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.
KLH-conjugated synthetic peptide of AMY derived from Oryza sativa AAA33885.1.
Protein A purified
Reconstitution with 150 µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa, Zea mays, and 80-99% homologues with the sequence in Gossypium raimondii, Triticum aestivum, Glycine max, Medicago truncatula, Solanum tuberosum, Solanum lycopersicum. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 10 μg total protein from Hordeum vulgare leaf . Lane 2: 25 μg total protein from Hordeum vulgare leaf. Lane 3: 50 μg total protein from Hordeum vulgare leaf. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.