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PsaA / Anti-Photosystem I P700 chlorophyll a apoprotein A1 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY5260S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX.

  Synonyms:

PsaA

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of PsaA protein in Synechocystis sp. PCC 6803 (slr1834)

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

83 / 60 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Oryza sativa Japonica Group, Chlamydomonas reinhardtii, Arabidopsis thaliana, Populus trichocarpa, Spinacia oleracea, Nicotiana tabacum, Triticum aestivum, Cucumis sativus, Zea mays.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 50 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803
Electrophoresis: 12.5% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1.5 h.
Blocking: 5% skim milk at RT or 4℃ for 2 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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