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PsbA / Anti-Photosystem II protein D1 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY5268S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem II (PSII) is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.

  Synonyms:

PsbA

  Immunogen:

KLH-conjugated synthetic peptide of PsbA derived from Synechocystis sp. PCC 6803(slr1311)

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

40 / 30 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Nicotiana tabacumArabidopsis thalianaBrassica napusSetaria viridisOryza sativaMedicago truncatulaVitis viniferaGossypium raimondiiGlycine maxTriticum aestivumHordeum vulgareSynechococcus elongatus PCC 7942Chlamydomonas reinhardtiiSolanum lycopersicumZea maysPhyscomitrella patensPopulus trichocarpaPanicum virgatumSpinacia oleraceaCucumis sativus.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 15 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803
Lane 2: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1.5 h.
Blocking: 5% skim milk at RT or 4℃ for 2 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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