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CrGUN4 / Anti-Tetrapyrrole-binding protein antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY5504S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Tetrapyrrole-binding protein, chloroplast precursor. In Arabidopsis, GUN4 (Genomes uncoupled 4) is required for the functioning of the plastid mediated repression of nuclear transcription that is involved in controlling the levels of magnesium- protoporph.

  Synonyms:

CrGUN4

  Immunogen:

Recombinant protein of CrGUN4ΔTP(Chlamydomonas reinhardtii GUN4 protein without chloroplast transit peptides, amino acids 53-260).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

29 kDa

  Confirmed Reactivity: 

Chlamydomonas reinhardtii and Anti-GUN4 recognizes recombinant protein CrGUN4ΔTP, mature CrGUN4 and CrGUN4 fused with tag.

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 30 μg total Nicotiana benthamiana leaf protein. 
Lane 2: 30 μg total Arabidopsis thaliana leaf protein.
Lane 3: 30 μg total Glycine max leaf protein. 
Lane 4: 30 μg total Lotus japonicus leaf protein 
Lane 5: 30 μg total Chlamydomonas reinhardtii protein.
Lane 6: 5 ng His-CrGUN4△TP fusion protein.
Lane 7: 1 ng His-CrGUN4△TP fusion protein.
Lane 8: 0.1 ng His-CrGUN4△TP fusion protein.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to PVDF membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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