Product Information
Description: | Rabbit polyclonal antibody |
Background: | ATPase beta subunit, which is a subunit of ATP synthase and part of the CF1 portion which catalyzes the conversion of ADP to ATP using the proton motive force. This complex is located in the thylakoid membrane of the chloroplast. |
Synonyms: | AtpB, ATP synthase subunit beta, chloroplastic, ATP synthase F1 sector subunit beta, F-ATPase subunit beta. |
Immunogen: | KLH-conjugated synthetic peptide of AtpB derived from Arabidopsis thaliana ATCG00480. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 53.9 / 56 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Medicago truncatula, Triticum aestivum, Populus trichocarpa, Solanum tuberosum, Glycine max, Solanum tuberosum, Oryza sativa Indica Group, Hordeum vulgare, Spinacia oleracea, Zea mays, Solanum lycopersicum, Setaria viridis, Gossypium raimondii, Panicum virgatum, Sorghum bicolor, Vitis vinifera, Cucumis sativus, Physcomitrella patens, Synechocystis sp. PCC 6803, Nicotiana tabacum, and 80-99% homologues with the sequence in Chlamydomonas reinhardtii. |
Application Example
Example 1
1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.25μg, 0.5μg, and 0.75μg of chlorophyll, respectively. |