AraShare科学

Product Information

Description:	Rabbit polyclonal antibody
Background:	ATPase beta subunit, which is a subunit of ATP synthase and part of the CF1 portion which catalyzes the conversion of ADP to ATP using the proton motive force. This complex is located in the thylakoid membrane of the chloroplast.
Synonyms:	AtpB, ATP synthase subunit beta, chloroplastic, ATP synthase F1 sector subunit beta, F-ATPase subunit beta.
Immunogen:	KLH-conjugated synthetic peptide of AtpB derived from Arabidopsis thaliana ATCG00480.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150 µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Example

Example 1

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.25μg, 0.5μg, and 0.75μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	53.9 / 56 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Medicago truncatula, Triticum aestivum, Populus trichocarpa, Solanum tuberosum, Glycine max, Solanum tuberosum, Oryza sativa Indica Group, Hordeum vulgare, Spinacia oleracea, Zea mays, Solanum lycopersicum, Setaria viridis, Gossypium raimondii, Panicum virgatum, Sorghum bicolor, Vitis vinifera, Cucumis sativus, Physcomitrella patens, Synechocystis sp. PCC 6803, Nicotiana tabacum, and 80-99% homologues with the sequence in Chlamydomonas reinhardtii. The sequence of the synthetic peptide used for immunization is also 93% homologues with the sequence in mitochondrial ATP synthase beta subunit At5g08670, At5g08680, At5g08690. For more species homologues information, please contact tech support at tech@phytoab.com.