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Arf1 /Anti-ADP-ribosylation factor 1 antibody
价格:¥2600.00 ¥2890.00
积分:130 分
货号:PHY0006S
数量:19998
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Arf1 is essential for vesicle coating and uncoating and functions in GTP-binding. It plays a role in cell division, cell expansion and cellulose production using antisense construct.

  Synonyms:

Arf1, AtARF1, ARF1A1C, BEX1, BFA-VISUALIZED EXOCYTIC TRAFFICKING DEFECTIVE 1

  Immunogen:

Recombinant, full length of Arf1 derived from Arabidopsis thaliana AT2G47170.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Example

Lane 1: 25 µg total protein from Arabidopsis thaliana leaf.
Lane 2: 50 µg total protein from Arabidopsis thaliana leaf.
Lane 3: 100 µg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

21 / 19 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

The sequence of the recombinant protein used for immunization is 98%-99% homologues with the sequence in ARFA1A (AT1G23490), ARFA1B (AT5G14670), ARFA1D (AT1G70490), ARFA1E (AT3G62290), ARFA1F (AT1G10630).
For more species homologues information, please contact tech support at tech@phytoab.com


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