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TIC20-I / Anti-Protein TIC20-I, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0451S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

TIC20 is a key component of the protein import machinery at the inner envelope membrane of chloroplasts. It is involved in protein precursor import into chloroplasts. The Arabidopsis genome encodes four Tic20 homologous proteins, AT1G04940(Tic20-I), AT2G47840(Tic20-II), AT4G03320(Tic20-IV) and AT5G55710(Tic20-V).

  Synonyms:

TIC20, ATTIC20, ATTIC20-I, TIC20-I, TRANSLOCON AT THE INNER ENVELOPE MEMBRANE OF CHLOROPLASTS 20

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal section of TIC20 protein in Arabidopsis thaliana AT1G04940.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

31 / 21 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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