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ERD10 / Anti-Dehydrin ERD10, C-terminal antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:P5988449052153
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Dehydrin (DHN) is a multi-family of proteins present in plants that is produced in response to cold and drought stress. ERD10 is induced by low temperature and dehydration and plays roles both in protection of the plants from various stresses, including cold and dehydration.

  Synonyms:

ERD10

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of ERD10 in Arabidopsis thaliana AT1G20450.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Example

Lane 1: 50 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 100 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 10 μg soluble protein from Arabidopsis thaliana leaf.
Lane 4: 15 μg soluble protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

29.5 / 31 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.


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