AraShare科学

Product Information

Description:	Rabbit polyclonal antibody
Background:	Cytochrome f is a component of cytochrome b6/f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
Synonyms:	PetA, photosynthetic electron transfer A, Cyt f.
Immunogen:	KLH-conjugated synthetic peptide of PetA derived from Arabidopsis thaliana ATCG00540.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Protein A purified
Reconstitution:	Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Example

Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Example 2

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.25 μg and 0.5 μg of chlorophyll, respectively.
3 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of Cyt b6f complex containing 0.5 μg of chlorophyll.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	35 / 39 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Solanum tuberosum, Oryza sativa Japonica Group, Medicago truncatula, Populus trichocarpa, Glycine max, Nicotiana tabacum, Zea mays, Spinacia oleracea, Vitis vinifera, Triticum aestivum, Gossypium raimondii, Panicum virgatum, Sorghum bicolor, Hordeum vulgare subsp. Vulgare, Cucumis sativus, Physcomitrella patens, Chlamydomonas reinhardtii. For more species homologues information, please contact tech support at tech@phytoab.com.