AraShare科学

Product Information

Description:	Rabbit polyclonal antibody
Background:	The D1 protein of Photosystem II (PSII), encoded by the psbA genes, is a subunit of PSII reaction center and is an indispensable component of oxygenic photosynthesis. Due to strongly oxidative chemistry of PSII water splitting, the D1 protein is prone to constant photodamage requiring its replacement, whereas most of the other PSII subunits remain ordinarily undamaged.
Synonyms:	PsbA, 32 kDa thylakoid membrane protein, photosystem II protein D1
Immunogen:	KLH-conjugated synthetic peptide of PsbA DE-loop derived from Arabidopsis thaliana ATCG00020.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Protein A purified
Reconstitution:	Reconstitution with 150 µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Example

Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Example 2

Line 1-2 is thylakoid membrane protein from Synechocystis sp. PCC 6803 cells containing 1μg and 2 μg of protein, respectively.
Electrophoresis: 12% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:5000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	39 / 31 kDa (Arabidopsis thaliana), 25 kDa (Synechocystis sp. PCC 6803)
Confirmed Reactivity:	Arabidopsis thaliana, Synechocystis sp. PCC 6803
Predicted Reactivity:	Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Glycine max, Medicago truncatula, Nicotiana tabacum, Populus trichocarpa, Brassica napus, Oryza sativa Japonica Group, Cucumis sativus, Chlamydomonas reinhardtii, Spinacia oleracea, Brassica rapa, and 80-99% homologues with the sequence in Zea mays, Triticum aestivum, Hordeum vulgare, Vitis vinifera, Panicum virgatum, Gossypium raimondii, Setaria viridis. For more species homologues information, please contact tech support at tech@phytoab.com.