Background:

Glutamine synthetase catalyses the synthesis of glutamine from glutamate and ammonia, which is one of the key enzymes involved in nitrogen metabolism of plants. Two classes of glutamine synthetase are present in plants, cytosolic form (GLN1) and chloroplastic form (GLN2). While GLN1 is encoded by five genes (AT5G37600, AT1G66200, AT3G17820, AT5G16570, AT1G48470), GLN2 is encoded by a single gene (AT5G35630).

  Synonyms:

GLN1/2.

  Immunogen:

KLH-conjugated synthetic peptide of GLN1 derived from Arabidopsis thaliana AT5G37600, AT1G66200, AT3G17820, AT1G48470.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Expected/apparent MW: 

39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Solanum lycopersicum, Spinacia oleracea, Zea mays, Oryza sativa Japonica Group, Nicotiana tabacum, Vitis vinifera, Brassica napus, Populus trichocarpa, Solanum tuberosum, and 80-99% homologues with the sequence in Triticum aestivum, Cucumis sativus, Gossypium raimondii, Hordeum vulgare.
The sequence of the synthetic peptide used for immunization is 86% homologues with the sequence in GLN2 (AT5G35630).
For more species homologues information, please contact tech support at tech@phytoab.com.