Background:

Ferredoxin-dependent glutamate synthase 1 (also known as Glutamine oxoglutarate aminotransferase) is an enzyme and frequently abbreviated as GOGAT. This enzyme manufactures glutamate from glutamine and α-ketoglutarate, and thus along with glutamine synthetase (abbreviated GS) plays a central role in the regulation of nitrogen assimilation in photosynthetic eukaryotes and prokaryotes. This is of great importance as primary productivity in many marine environments is regulated by the availability of inorganic nitrogen.
GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 (AT5G04140) and glu2 (AT2G41220) found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.

  Synonyms:

GLU1, FD-GOGAT, FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE, FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 1, GLS1, GLUS, GLUTAMATE SYNTHASE 1

  Immunogen:

KLH-conjugated synthetic peptide derived from central section of GLU1 protein in Arabidopsis thaliana AT5G04140.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Expected/apparent MW: 

180 / 170 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica rapa, Brassica napus.
The sequence of the synthetic peptide used for immunization is 81% homologues with the sequence in GLU2 (AT2G41220).
For more species homologues information, please contact tech support at tech@phytoab.com.