返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体
ChtC1 / Anti-Class I chitinase antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0018
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin.

  Synonyms:

ChtC1, ATHCHIB, B-CHI, BASIC CHITINASE, PR-3, PR3, CHI-B, HCHIB, PATHOGENESIS-RELATED 3.

  Immunogen:

KLH-conjugated synthetic peptide of ChtC1 derived from Solanum tuberosum NP_001275122.1.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

36 / 36 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana (93% homologues with the sequence in Arabidopsis thaliana AT3G12500).

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Solanum lycopersicum, Nicotiana tabacum, Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

10μg total protein from Arabidopsis thaliana leaf .
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.



猜你喜欢 CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00
CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00