Product Information
Description: | Rabbit polyclonal antibody |
Background: | Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants to energy-rich molecules such as glucose. In chemical terms, it catalyzes the carboxylation of ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most abundant enzyme on Earth. |
Synonyms: | RbcL, Ribulose-1,5-bisphosphate carboxylase, oxygenase |
Immunogen: | KLH-conjugated synthetic peptide of RbcL derived from Arabidopsis thaliana ATCG00490. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:4000-1:10000) |
Expected/apparent MW: | 53 / 53-55 kDa |
Confirmed Reactivity: | Arabidopsis thaliana, Spinacia oleracea |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Japonica Group, Leymus chinensis, Zea mays, Cucumis sativus, Gossypium raimondii, Hordeum vulgare subsp. Vulgare, Medicago truncatula, Brassica napus, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Triticum aestivum, Panicum virgatum, Vitis vinifera, Populus trichocarpa, Physcomitrella patens, Chlamydomonas reinhardtii, Glycine max, Setaria viridis. |
Application Example
Lane 1: 0.2μg soluble protein from Spinacia oleracea leaf . |