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HSP70 / Anti-Heat shock protein 70, mitochondrial antibody
价格:¥2310.00 ¥2890.00
积分:150 分
货号:PHY0034S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70, which exhibits both cytoprotectant and immunoregulatory functions. Members of the Hsp70 family are strongly upregulated by heat stress and toxic chemicals, particularly heavy metals such as arsenic, cadmium, copper, mercury, etc. Arabidopsis plants contain two genes coding for mitochondrial Hsp70-type chaperon-like proteins, AtHscA1 (At4g37910) and AtHscA2 (At5g09590).

  Synonyms:

HSP70, MTHSC70-1, AtHsp70-9, Heat shock protein 70-9

  Immunogen:

KLH-conjugated synthetic peptide of HSP70 derived from Arabidopsis thaliana AT4G37910.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

73 / 70 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

The sequence of the synthetic peptide used for immunization is 93% homologues with the sequence in mtHSC70-2(AT5g09590), ATHSP70-2(AT5G02490), HSP70-5(AT1G16030), HSP70-1(AT5G02500), HSP70-4(AT3G12580), HSP70-18(AT1G56410), and is 80% homologues with the sequence in cpHSC70-2(AT5G49910), cpHSC70-1(AT4G24280).
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis:10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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