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PsaD / Anti-PsaD subunit of photosystem I, N-terminal antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0056
数量:998
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem I (PSI) consists of at least eleven subunits in the thylakoid membrane, and mediates photosynthetic electron transfer from plastocyanin to ferredoxin. PsaD subunit, which is located at the stromal surface of the PSI, is thought to be the docking site of ferredoxin, and encoded by the nuclear gene PsaD-1(AT4G02770) and PsaD-2 (AT1G03130).

  Synonyms:

PsaD, PSI-D

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of PsaD in Arabidopsis thaliana.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

22.6 / 20 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Solanum lycopersicum, Solanum tuberosum, Brassica napus, and 80-99% homologues with the sequence in Gossypium raimondii, Glycine max, Medicago truncatula, Vitis vinifera, Oryza sativa Japonica Group, Spinacia oleracea, Populus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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