Product Information
Description: | Rabbit polyclonal antibody |
Background: | PsbS is a ubiquitous pigment-binding protein associated with photosystem II (PSII) of higher plants, which is involved in nonphotochemical quenching rather than in photosynthesis. Mutant has a normal violaxanthin cycle but has a limited capacity of quenching singlet excited chlorophylls and is tolerant to lipid peroxidation. |
Synonyms: | PsbS, 22 kDa Lhc-like PSII protein |
Immunogen: | KLH-conjugated synthetic peptide derived of PsbS from Arabidopsis thaliana AT1G44575. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 28 / 21 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Gossypium raimondii, Solanum lycopersicum, Physcomitrella patens, Vitis vinifera, Populus trichocarpa, Solanum tuberosum, Nicotiana tabacum, Cucumis sativus, Glycine max, Oryza sativa Japonica Group, Zea mays. |
Application Example
Example 1
1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. |
Example 2
1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.25 μg and 0.5 μg of chlorophyll, respectively. |