Product Information
Description: | Rabbit polyclonal antibody |
Background: | Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants to energy-rich molecules such as glucose. In chemical terms, it catalyzes the carboxylation of ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most abundant enzyme on Earth. |
Synonyms: | RbcL, Ribulose-1,5-bisphosphate carboxylase, oxygenase |
Immunogen: | KLH-conjugated synthetic peptide of RbcL derived from Arabidopsis thaliana ATCG00490. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Protein A purified |
Reconstitution: | Reconstitution with 150 µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 53 / 53-55 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Brassica rapa, Leymus chinensis, Setaria viridis, Cucumis sativus, Gossypium raimondii, Medicago truncatula, Brassica napus, Nicotiana tabacum, Solanum lycopersicum, Panicum virgatum, Triticum aestivum, Solanum tuberosum, Oryza sativa, Sorghum bicolor, Spinacia oleracea, Chlamydomonas reinhardtii, Populus trichocarpa, Glycine max, Physcomitrium patens, Vitis vinifera, Hordeum vulgare. |
Application Example
Lane 1: 5 μg soluble protein from Arabidopsis thaliana leaf. |