Product Information
Description: | Rabbit polyclonal antibody |
Background: | In photosynthetic organisms, D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the major enzyme assimilating atmospheric CO2 into the biosphere. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit (RbcL) is coded by the chloroplast rbcL gene, and the 15-kD small subunit (RbcS) is coded by a family of nuclear rbcS genes (AT1G67090, AT5G38430, AT5G38420, AT5G38410). |
Synonyms: | RbcS, SSU |
Immunogen: | KLH-conjugated synthetic peptide of RbcS derived from Arabidopsis thaliana AT1G67090. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Protein A purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:5000) |
Expected/apparent MW: | 20 / 15 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus, Brassica rapa, Populus trichocarpa. |
Application Example
Lane 1: 4μg total protein from Arabidopsis thaliana leaf . |