Product Information
Description: | Rabbit polyclonal antibody |
Background: | Two types of small GTP-binding proteins, ADP-ribosylation factor (Arf) and secretion-associated and Ras-related (Sar), are major regulators of vesicle biogenesis in intracellular traffic and are founding members of a growing family that also includes Arf-relatedproteins (Arp) and Arf-like (Arl) proteins. Sar1 functions as a molecular switch to control the assembly of protein coats (COPII) that direct vesicle budding from ER. |
Synonyms: | Sar1 |
Immunogen: | KLH-conjugated synthetic peptide of Sar1 derived from Arabidopsis thaliana AT3G62560. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 22 / 24 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Solanum tuberosum, and 80-99% homologues with the sequence in Populus trichocarpa. |
Application Example
Lane 1: 25 μg total protein from Arabidopsis thaliana leaf. |