返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体
RpoB / Anti-RNA polymerase subunit beta antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY1701
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Mouse monoclonal (Clone: 17P12) antibody

  Background:

In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β,β’,β’’) and a promoter recognition subunit (σfactor). RpoB (ATCG00190) is the β subunit of PEP.

  Synonyms:

RpoB, RNA POLYMERASE SUBUNIT BETA

  Immunogen:

Recombinant protein of RpoB derived from Arabidopsis thaliana ATCG00190.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Product Citations:

Ding S, et al., Molecular Plant, September 2019, Vol. 12, pp. 1259-1277.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

121 kDa, 60kDa (Recombinant protein)

  Confirmed Reactivity: 

Arabidopsis thaliana, Oryza sativa

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

 Example 1

Lane 1: 1 ng recombinant protein of RpoB.
Lane 2: 3 ug stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 6 ug stromal protein from Arabidopsis thaliana leaf. 
Lane 4: 0.5 ug thylakoid membrane protein from Arabidopsis thaliana leaf. 
Lane 5: 1 ug thylakoid membrane protein from Arabidopsis thaliana leaf. 
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP).
Detection: using chemiluminescence substrate and image were captured with CCD camera.

  Example 2

rice: 50 μg total protein from Oryza sativa leaf. 
Arab.: 100 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP).
Detection: using chemiluminescence substrate and image were captured with CCD camera.


猜你喜欢 CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00
CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00