Product Information
Description: | Mouse monoclonal (Clone: 17P12) antibody |
Background: | In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β,β’,β’’) and a promoter recognition subunit (σfactor). RpoB (ATCG00190) is the β subunit of PEP. |
Synonyms: | RpoB, RNA POLYMERASE SUBUNIT BETA |
Immunogen: | Recombinant protein of RpoB derived from Arabidopsis thaliana ATCG00190. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Protein A purified |
Reconstitution: | Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Product Citations: | Ding S, et al., Molecular Plant, September 2019, Vol. 12, pp. 1259-1277. |
Application Information
Applications: | Western Blot (1:1000-1:2000) |
Expected Results: | 121 kDa, 60kDa (Recombinant protein) |
Confirmed Reactivity: | Arabidopsis thaliana, Oryza sativa |
Predicted Reactivity: | For more species homologues information, please contact tech support at arashare01@qq.com. |
Application Example
Example 1
Lane 1: 1 ng recombinant protein of RpoB. |
Example 2
rice: 50 μg total protein from Oryza sativa leaf. |