Product Information
Description: | Rabbit polyclonal antibody |
Background: | In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclearencoded RNA polymerase (NEP). Transcription in plastids is also mediated by a number of nuclear-encoded factors in addition to PEP and NEP. In the insoluble RNA polymerase preparation samples, a total of 18 components named as pTACs (pTAC1 to pTAC18) were identified. pTAC3 (AT3G04260) is one of the components associated with PEP complex. |
Synonyms: | pTAC3, PDE324, PIGMENT DEFECTIVE 324, PLASTID TRANSCRIPTIONALLY ACTIVE 3, PTAC3 |
Immunogen: | KLH-conjugated synthetic peptide derived from central section of pTAC3 protein in Arabidopsis thaliana AT3G04260. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:5000) |
Expected/apparent MW: | 103 kDa |
Confirmed Reactivity: | Arabidopsis thaliana, Zea mays, Oryza sativa |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Japonica Group, Gossypium raimondii, Spinacia oleracea, Populus trichocarpa, Zea mays, Glycine max, Setaria viridis, Sorghum bicolor, Hordeum vulgare, and 80-99% homologues with the sequence in Brassica rapa, Brassica napus, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera, Nicotiana tabacum, Cucumis sativus, Medicago truncatula. |
Application Example
Lane 1: 5 μl total chloroplast protein from Arabidopsis thaliana leaf. |