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PsbO / Anti-PsbO subunit of oxygen evolving complex of Photosystem II antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0094A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbO is an extrinsic subunit of photosystem II, which has been proposed to play a central role in stabilization of the catalytic manganese cluster. In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: psbO1 (AT5G66570) and psbO2(AT3G50820). PsbO1 is the major isoform in Arabidopsis.

  Synonyms:

PsbO, OEE1, 33 kDa subunit of oxygen evolving system of photosystem II, 33 kDa thylakoid membrane protein, Manganese-stabilizing protein 1, MSP-1, OEC 33 kDa subunit, 33 kDa of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide of PsbO derived from Arabidopsis thalianaAT5G66570, AT3G50820.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

35 / 33-35 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patens, Nicotiana tabacum, Brassica napus, Vitis vinifera, Zea mays, Oryza sativa Japonica Group, Solanum tuberosum, Setaria viridis, Brassica rapa, Solanum lycopersicum, Cucumis sativus, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Populus trichocarpa, Gossypium raimondii, Leymus chinensis, Hordeum vulgare, Chlamydomonas reinhardtii, and 80-99% homologues with the sequence in Glycine max, Medicago truncatula.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.5μg, 1μg, and 2μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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