Product Information
Description: | Rabbit polyclonal antibody |
Background: | PsbO is an extrinsic subunit of photosystem II, which has been proposed to play a central role in stabilization of the catalytic manganese cluster. In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: psbO1 (AT5G66570) and psbO2(AT3G50820). PsbO1 is the major isoform in Arabidopsis. |
Synonyms: | PsbO, OEE1, 33 kDa subunit of oxygen evolving system of photosystem II, 33 kDa thylakoid membrane protein, Manganese-stabilizing protein 1, MSP-1, OEC 33 kDa subunit, 33 kDa of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 1, chloroplastic |
Immunogen: | KLH-conjugated synthetic peptide of PsbO derived from Arabidopsis thalianaAT5G66570, AT3G50820. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:5000) |
Expected/apparent MW: | 35 / 33-35 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patens, Nicotiana tabacum, Brassica napus, Vitis vinifera, Zea mays, Oryza sativa Japonica Group, Solanum tuberosum, Setaria viridis, Brassica rapa, Solanum lycopersicum, Cucumis sativus, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Populus trichocarpa, Gossypium raimondii, Leymus chinensis, Hordeum vulgare, Chlamydomonas reinhardtii, and 80-99% homologues with the sequence in Glycine max, Medicago truncatula. |
Application Example
1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.5μg, 1μg, and 2μg of chlorophyll, respectively. |