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PsbO / Anti-PsbO subunit of oxygen evolving complex of Photosystem II antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0094A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbO is an extrinsic subunit of photosystem II, which has been proposed to play a central role in stabilization of the catalytic manganese cluster. In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: psbO1 (AT5G66570) and psbO2(AT3G50820). PsbO1 is the major isoform in Arabidopsis.

  Synonyms:

PsbO, OEE1, 33 kDa subunit of oxygen evolving system of photosystem II, 33 kDa thylakoid membrane protein, Manganese-stabilizing protein 1, MSP-1, OEC 33 kDa subunit, 33 kDa of the oxygen evolving complex (OEC) of PSII, Oxygen-evolving enhancer protein 1, chloroplastic

  Immunogen:

KLH-conjugated synthetic peptide of PsbO derived from Arabidopsis thalianaAT5G66570, AT3G50820.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

35 / 33-35 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patensNicotiana tabacumBrassica napusVitis viniferaZea maysOryza sativa Japonica GroupSolanum tuberosumSetaria viridisBrassica rapaSolanum lycopersicumCucumis sativusSorghum bicolorSpinacia oleraceaTriticum aestivumPopulus trichocarpaGossypium raimondiiLeymus chinensisHordeum vulgareChlamydomonas reinhardtii, and 80-99% homologues with the sequence in Glycine maxMedicago truncatula.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.5μg, 1μg, and 2μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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