Product Information
Description: | Rabbit polyclonal antibody |
Background: | NAD-dependent isocitrate dehydrogenase (IDH) is a Krebs cycle enzyme situated in mitochondria. In Arabidopsis thaliana, five genes encode functional IDH subunits that can be classed into two groups based on gene structure and subunit amino acid sequence. Arabidopsis contains two 'catalytic' and three 'regulatory' subunits according to their homology with yeast IDH. IDH1 performs an essential role in the oxidative function of the citric acid cycle. |
Synonyms: | IDH1, IDH-I, ISOCITRATE DEHYDROGENASE 1, ISOCITRATE DEHYDROGENASE I |
Immunogen: | KLH-conjugated synthetic peptide of IDH1 derived from Arabidopsis thalianaAT4G35260. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 40 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Cucumis sativus, Spinacia oleracea, Setaria viridis, Vitis vinifera, Populus trichocarpa, Gossypium raimondii, Glycine max, Brassica napus, and 80-99% homologues with the sequence in Oryza sativa Japonica Group, Zea mays, Sorghum bicolor, Hordeum vulgare, Solanum lycopersicum, Nicotiana tabacum, Solanum tuberosum. |
Application Example
10 μg mitochondria protein from Arabidopsis thaliana leaf. |