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PRK / Anti-Phosphopentokinase antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0100A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Phosphoribulokinase is an enzyme that catalyzes ATP and D-ribulose 5-phosphate to produce ADP and D-ribulose 1,5-bisphosphate. This protein is involved in the pathway Calvin cycle.

  Synonyms:

PRK, PRK RIBULOSE-5-P-KINASE, PRKase.

  Immunogen:

KLH-conjugated synthetic peptide of PRK derived from Arabidopsis thalianaAT1G32060.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

44.4 / 44 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea may, Medicago truncatula, Populus trichocarpa, Triticum aestivum, Synechococcus elongatus PCC 7942, Synechocystis sp. PCC 6803, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

10μg total protein from Arabidopsis thaliana leaf . 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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