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EF1A / Anti-Elongation factor 1-alpha antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0101A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

  Synonyms:

EF1A

  Immunogen:

KLH-conjugated synthetic peptide of EF1A derived from  Arabidopsis thalianaAT1G07940.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

49.5 / 50 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Medicago truncatula, Gossypium raimondii, Brassica rapa, Oryza sativa Japonica Group, Solanum tuberosum, Solanum lycopersicum, Glycine max, Hordeum vulgare, Nicotiana tabacum, Zea mays.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 25 μg total protein from Arabidopsis thaliana leaf . 
Lane 2: 50 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.