Product Information
Description: | Rabbit polyclonal antibody |
Background: | EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
Synonyms: | EF1A |
Immunogen: | KLH-conjugated synthetic peptide of EF1A derived from Arabidopsis thalianaAT1G07940. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 49.5 / 50 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Medicago truncatula, Gossypium raimondii, Brassica rapa, Oryza sativa Japonica Group, Solanum tuberosum, Solanum lycopersicum, Glycine max, Hordeum vulgare, Nicotiana tabacum, Zea mays. |
Application Example
Lane 1: 25 μg total protein from Arabidopsis thaliana leaf . |