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RbcL / Anti-Rubisco large subunit, form I antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0104
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants to energy-rich molecules such as glucose. In chemical terms, it catalyzes the carboxylation of ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most abundant enzyme on Earth. 
The enzyme usually consists of two types of protein subunit, called the large chain (RbcL) and the small chain (RbcS).

  Synonyms:

RbcL, Ribulose bisphosphate carboxylase large chain, Ribulose-1,5-bisphosphate carboxylase, oxygenase

  Immunogen:

KLH-conjugated synthetic peptide of RbcL derived from Arabidopsis thalianaATCG00490.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

55 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. Chinensis, Leymus chinensis, Oryza sativa Indica Group, Zea mays, Cucumis sativus, Gossypium raimondii, Hordeum vulgare subsp. Vulgare, Medicago truncatula, Gossypium raimondii, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Triticum aestivum, Panicum virgatum, Cucumis sativus, Sorghum bicolor, Leymus chinensis, Vitis vinifera, Populus trichocarpa, Physcomitrella patens, Spinacia oleracea, Chlamydomonas reinhardtii, Glycine max, Setaria viridis, Brassica napus, Brassica rapa, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 0.1375 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 0.275 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 0.3575 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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