Product Information
Description: | Rabbit polyclonal antibody |
Background: | The CFoIII subunits of chloroplast ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains. |
Synonyms: | AtpH, ATP synthase F(0) sector subunit c, Lipid-binding protein Dicyclohexylcarbodiimide-binding protein, F-type ATPase subunit c, F-ATPase subunit c |
Immunogen: | KLH-conjugated synthetic peptide of AtpH derived from Arabidopsis thalianaATCG00140. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 8 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Oryza sativa Japonica Group, Glycine max, Nicotiana tabacum, and 80-99% homologues with the sequence in Physcomitrella patens, Chlamydomonas reinhardtii. |
Application Example
Example 1
1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.25μg, 0.5μg, and 1μg of chlorophyll, respectively. |
Example 2
1-3 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1 μg, 2.5 μg and 5 μg of chlorophyll, respectively. |