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PsaF / Anti-PsaF subunit of Photosystem I antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0120S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsaF participates in efficiency of electron transfer from plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria).

  Synonyms:

PsaF, PHOTOSYSTEM I SUBUNIT F, PSAF

  Immunogen:

KLH-conjugated synthetic peptide of PsaF derived from Arabidopsis thalianaAT1G31330.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 / 17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Spinacia oleracea, Oryza sativa Japonica Group, Zea mays, Solanum lycopersicum, Populus trichocarpa, Medicago truncatula, Glycine max.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.5 μg of chlorophyll. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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