Product Information
Description: | Rabbit polyclonal antibody |
Background: | Deg1 is a nuclear gene encoding chloroplast-targeted protease that can degrade two lumenal proteins, plastocyanin and OE33, suggesting a role as a general-purpose protease in the thylakoid lumen. It is involved in the degradation of D1 protein of PS II, hence participating in the repair of PS II damages caused by photoinhibition. |
Synonyms: | Deg1, DEG1, DEGP PROTEASE 1, DEGP1, DEGRADATION OF PERIPLASMIC PROTEINS 1 |
Immunogen: | KLH-conjugated synthetic peptide of Deg1 derived from Arabidopsis thalianaAT3G27925. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 47 / 36 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Spinacia oleracea, Zea mays, Setaria viridis, Brassica rapa, Cucumis sativus, Oryza sativa Japonica Group, Brassica napus, Chlamydomonas reinhardtii, Gossypium raimondii, Sorghum bicolor, Medicago truncatula, Nicotiana tabacum, Solanum tuberosum, Solanum lycopersicum, Populus trichocarpa, Hordeum vulgare, Glycine max, and 80-99% homologues with the sequence in Physcomitrium patens. |
Application Example
80 μg total chloroplast protein from Arabidopsis thaliana leaf. |