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Deg1 / Anti-Chloroplastic DegP-type serine protease 1 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0146A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Deg1 is a nuclear gene encoding chloroplast-targeted protease that can degrade two lumenal proteins, plastocyanin and OE33, suggesting a role as a general-purpose protease in the thylakoid lumen. It is involved in the degradation of D1 protein of PS II, hence participating in the repair of PS II damages caused by photoinhibition.

  Synonyms:

Deg1, DEG1, DEGP PROTEASE 1, DEGP1, DEGRADATION OF PERIPLASMIC PROTEINS 1

  Immunogen:

KLH-conjugated synthetic peptide of Deg1 derived from Arabidopsis thalianaAT3G27925.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

47 / 36 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Spinacia oleracea, Zea mays, Setaria viridis, Brassica rapa, Cucumis sativus, Oryza sativa Japonica Group, Brassica napus, Chlamydomonas reinhardtii, Gossypium raimondii, Sorghum bicolor, Medicago truncatula, Nicotiana tabacum, Solanum tuberosum, Solanum lycopersicum, Populus trichocarpa, Hordeum vulgare, Glycine max, and 80-99% homologues with the sequence in Physcomitrium patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

80 μg total chloroplast protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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