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GLN2 / Anti-GS2, chloroplastic form of glutamine synthetase antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0157
数量:999
- +
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Glutamine synthetase catalyses the synthesis of glutamine from glutamate and ammonia, which is one of the key enzymes involved in nitrogen metabolism of plants. Two classes of glutamine synthetase are present in plants, cytosolic form (GLN1) and chloroplastic form (GLN2). While GLN1 is encoded by five genes (AT5G37600, AT1G66200, AT3G17820, AT5G16570, AT1G48470), GLN2 is encoded by a single gene (AT5G35630).

  Synonyms:

GLN2, ATGSL1, GLUTAMINE SYNTHETASE 2, GLUTAMINE SYNTHETASE LIKE 1, GS2

  Immunogen:

KLH-conjugated synthetic peptide of GLN2 derived from Arabidopsis thalianaAT5G35630.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Indica Group, Zea mays, Brassica napus, Brassica rapa, Triticum aestivum, Vitis vinifera, Gossypium raimondii, Nicotiana tabacum, Populus trichocarpa, Glycine max, Solanum lycopersicum, Cucumis sativus, Spinacia oleracea, Medicago truncatula. 
The sequence of the synthetic peptide used for immunization is 85% homologues with the sequence in GLN1;1- GLN1;3 (AT5G37600, AT1G66200, AT3G17820), 92% in GLN1;4 (AT5G16570), and 78% in GLN1;5 (AT1G48470).
 For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 15 μg total soluble protein from Arabidopsis thaliana leaf. 
Lane 2: 30 μg total soluble protein from Arabidopsis thaliana leaf. 
Lane 3: 45 μg total soluble protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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