Product Information
Description: | Rabbit polyclonal antibody |
Background: | Glutamine synthetase catalyses the synthesis of glutamine from glutamate and ammonia, which is one of the key enzymes involved in nitrogen metabolism of plants. Two classes of glutamine synthetase are present in plants, cytosolic form (GLN1) and chloroplastic form (GLN2). While GLN1 is encoded by five genes (AT5G37600, AT1G66200, AT3G17820, AT5G16570, AT1G48470), GLN2 is encoded by a single gene (AT5G35630). |
Synonyms: | GLN2, ATGSL1, GLUTAMINE SYNTHETASE 2, GLUTAMINE SYNTHETASE LIKE 1, GS2 |
Immunogen: | KLH-conjugated synthetic peptide of GLN2 derived from Arabidopsis thalianaAT5G35630. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Protein A purified |
Reconstitution: | Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form) |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Indica Group, Zea mays, Brassica napus, Brassica rapa, Triticum aestivum, Vitis vinifera, Gossypium raimondii, Nicotiana tabacum, Populus trichocarpa, Glycine max, Solanum lycopersicum, Cucumis sativus, Spinacia oleracea, Medicago truncatula. |
Application Example
Lane 1: 15 μg total soluble protein from Arabidopsis thaliana leaf. |