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PGR5 / Protein PROTON GRADIENT REGULATION 5, chloroplastic antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0340A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Protein PROTON GRADIENT REGULATION 5 is involved in the regulation of the cyclic electron flow (CEF) around Photosystem I. It is essential for photoprotection.

  Synonyms:

PGR5, ATPGR5, PROTON GRADIENT REGULATION 5

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of of PGR5 protein in Arabidopsis thaliana AT2G05620

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

14 / 10 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Vitis vinifera, Populus trichocarpa, and 80-99% homologues with the sequence in Cucumis sativus, Spinacia oleracea, Oryza sativa Japonica Group, Medicago truncatula, Zea mays, Triticum aestivum, Hordeum vulgare, Sorghum bicolor, Solanum lycopersicum.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-2 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 2.5 μg and 5 μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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