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PGRL1A / Anti-PGR5-like protein 1A, chloroplastic antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0341
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

In higher plants, cyclic electron flow (CEF) around photosystem I contains two pathways, NDH-dependent and PGR5/PGRL1-dependent pathway. PGR5-like protein 1 (PGRL1) is one of the component that is essential for cyclic electron flow (CEF) around Photosystem I.

  Synonyms:

PGRL1A

  Immunogen:

Recombinant protein of PGRL1A (1-324aa) derived from Arabidopsis thaliana AT4G22890.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

36 / 28 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example  

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.25 μg and 0.5 μg of chlorophyll, respectively.
3 is thylakoid membrane protein from Arabidopsis pgrl1 mutant containing 0.5 μg of chlorophyll.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.